关键词: 小鼠, 急性酒精中毒, 肝损伤, 转棒行为学, 美他多辛

Abstract: Objective To investigate the value of rotarod test in treating acute alcoholism and the efficacy of metadoxine in acute alcoholism mice. Method Thirty male C57 mice were randomly divided into three groups: normal control group, model control group, and metadoxine treatment group [ 0. 3 mg / 10 ( g· bw) ] . After oral administration of metadoxine or saline, mice were given 0. 15 mL / 10 ( g· bw) of 56° Erguotou for one hour. The timeframe of intoxication and recovery from intoxication in mice were documented. 6 hours after alcohol administration, rotarod tests were conducted, blood was collected to measure the levels of serum aspartate aminotransferase ( AST) , alanine aminotransferase ( ALT) , and triglyceride ( TG) in mice. Additionally, the activity of alcohol dehydrogenase ( ADH ) , superoxide dismutase ( SOD) and catalase ( CAT) , malondialdehyde ( MDA) content in the liver were measured, and hematoxylin-eosin ( HE) staining was used to observe histopathology changes in the liver tissue. Result Following alcohol gavage, the model control group demonstrated a shorter period of intoxication and a longer period of sobriety. The rotarod behavior test revealed that the rod rotation time was significantly shortened, the rotational speed was reduced upon falling, and the number of rod laps was reduced. Additionally, serum AST, ALT, and TG were significantly elevated, the activity of liver ADH and SOD were significantly decreased, and MDA content was significantly increased; all of which were significantly different than the normal control group ( P < 0. 01) . Histological analysis via HE staining showed necrosis and congestion in the liver. In comparison to the control group, metadoxine treatment group mice exhibited a longer period of intoxication, a quicker rate of sobriety, a higher rotational speed during rotarod test, and a significantly heightened serum AST, ALT, TG, and liver ADH. MDA levels were decreased and the differences were highly statistically significant. The HE staining indicated that the liver lesions showed regional characteristics. The degree of liver cell damage was significantly reduced compared to the control group, and no obvious lesions were observed in some liver areas. Conclusion Rotarod test can be employed to assess acute alcoholism in mice, and metadoxine has been found to provide a protective effect against acute alcohol intoxication. 

Key words: mice, acute alcohol intoxication, liver damage, rotation behavior, metadoxine